Subtle messages about unconditional friendship, loyalty, tolerance, and what constitutes a family. The Pirate Captain's crew is indeed a bunch of misfits (an albino, a woman pretending to be a man, a peg-legged pirate, and a captain who can't seem to find any booty), but they stand by one another, even when times are dire.
Some gun, sword, and hand-to-hand violence. There are explosions and close calls, but no one is killed or permanently injured, although the queen must be subdued in order to save the Dodo bird that was going to be slaughtered and eaten. The pirates face off against ghosts.
Female pirate Cutlass Liz has noticeable cleavage, and the male pirates stare at her curves in one saloon scene. Darwin has an overwhelming crush on the queen and says her severe bun "does it for him." The Pirate Captain flirts with one of Darwin's female friends during a night at the pub. The "Surprisingly Curvaceous Pirate" is actually a woman, and she ends up in a tub, but nothing is ever shown.
Language includes infrequent use of "hell," "ass," and "crap," plus "idiot" and "stupid."
Drinking, Drugs & Smoking
The pirates are often shown at a saloon or celebrating the Pirate of the Year Awards, so there's a noticeable amount of drinking (what looks like beer, wine, or mead/grog). The Pirate Captain gets drunk hanging out with Charles Darwin and his friends.