A primary theme is that things are not always as they appear and people are not always who they seem to be. The mix of sexuality and animation in Jessica Rabbit's character might be confusing to some kids.

Several scenes feature cartoon violence that borders on the sadistic and the surreal. Characters are thrown into "the dip" (an acid-like concoction that will "erase" toons), a live-action character is shot on screen, and Judge Doom is run over by a steam engine.

Silly double entendres proliferate, including character Baby Herman's reference to his inability to pursue his adult nurse being the result of having "50-year-old lust and a 3-year-old dinky." Jessica Rabbit has a voluptuous figure and wears very revealing clothes. Everything she does exudes sexiness, even when playing "patty-cake" with a director.

Adult language used by the live-action characters includes "son of a bitch" and "bastard." Derogatory remarks are made against the toons.

Classic Chevrolets line the streets, Acme labels are lurking around every corner, along with Wild Turkey whiskey, the main character's favorite drink.

The main character is an alcoholic and drinks heavily throughout the day. Smoking is also prevalent.