The Sanctuary is a mysterious organization that exists outside standard law enforcement channels, and though Dr. Zimmerman sometimes crosses paths with his former colleagues on the police force, he must keep his true activities and motives secret. The team sometimes deals with black market weapons brokers. The most dangerous creatures are locked up deep within the Sanctuary; some are willing to stay there for their own protection, while others pose a danger to society and are held against their will.
Some of the abnormals are benign, but many are quite dangerous, and their attacks can be violent, deadly, and gory. Though the Sanctuary staff has no desire to hurt these creatures, they're often forced to subdue their quarry using stun-guns or darts with sleep-inducing drugs. Ashley is often heavily armed; she's quick with both lethal and non-lethal weapons and sometimes engages in martial arts combat.
No nudity or sex, though there is some flirting between Will and Ashley.